ZORBAX GF-250/450

Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series.

The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.

The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.

Common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.

Aggregate & Fragment Analysis

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Product List

1 - 12 of 14 results

Filter by Inner Diameter (ID) 4.6 mm 9.4 mm 17 mm 21.2 mm Length 8 mm 12.5 mm 13 mm 15 mm 250 mm Particle Size 4 µm 5 µm 6 µm Pore Size 150 Å 300 Å Guard Column Yes Phase GF-250 GF-250 Diol GF-450 Pressure Rating 135 bar 400 bar

									QTY
420420-901	Agilent Prep External Guard Hardware Kit						1	Request Quote	Quantity
Agilent Prep External Guard Hardware Kit See all product information Est. ship date 4 July
820999-901	High performance ZORBAX guard fittings kit (ZGC)	Kit	4.6 mm	12.5 mm			1 Each	Request Quote	Quantity
High performance ZORBAX guard fittings kit (ZGC); includes low-volume guard holder, inlet end fitting (2), outlet end fitting with integrated column connector, and PEEK fingertight fitting, seals (2/pk) Guard Column: Yes Hardware: Kit Inner Diameter (ID): 4.6 mm Length: 12.5 mm See all product information
820400-901	PrepHT cartridge hardware kit, 21.2 mm end fittings, 2/pk		21.2 mm				2 Pack	Request Quote	Quantity
PrepHT cartridge hardware kit, 21.2 mm end fittings, 2/pk Inner Diameter (ID): 21.2 mm See all product information
820444-901	PrepHT Internal Guard Cartridge Hardware Kit						1	Request Quote	Quantity
PrepHT Internal Guard Cartridge Hardware Kit See all product information
840140-901	Semi-prep guard cartridge hardware kit, 9.4 mm ID cartridges (P system)		9.4 mm	15 mm			1 Kit	Request Quote	Quantity
Semi-prep guard cartridge hardware kit, 9.4 mm ID cartridges (P system) Guard Column: Yes Inner Diameter (ID): 9.4 mm Length: 15 mm See all product information
820950-911	ZORBAX Diol, 4.6 x 12.5 mm, 6 µm, guard cartridge (ZGC), 4/pk		4.6 mm	13 mm	6 µm		4 Pack	Request Quote	Quantity
ZORBAX Diol, 4.6 x 12.5 mm, 6 µm, guard cartridge (ZGC), 4/pk. Use with hardware kit 820999-901 Guard Column: Yes Inner Diameter (ID): 4.6 mm Length: 13 mm Particle Size: 6 µm See all product information Est. ship date 4 July
820675-111	ZORBAX Diol, 9.4 x 15 mm, 6 µm, guard cartridge (P), 2/pk	Cartridge	9.4 mm	15 mm	6 µm	GF-250 Diol	2 Pack	Request Quote	Quantity
ZORBAX Diol, 9.4 x 15 mm, 6 µm, guard cartridge (P), 2/pk (use with hardware kit 840140-901) Phase: GF-250 Diol Guard Column: Yes Molecular Weight Lower Limit: 0 Da Molecular Weight Upper Limit: 3000 Da Hardware: Cartridge Inner Diameter (ID): 9.4 mm Length: 15 mm Particle Size: 6 µm See all product information Est. ship date 4 July
877974-901	ZORBAX GF-250, 21.2 x 250 mm, 6 µm, cartridge (PrepHT)	SS	21.2 mm	250 mm	6 µm	GF-250	1 Each	Request Quote	Quantity
ZORBAX GF-250, 21.2 x 250 mm, 6 µm, cartridge (PrepHT). Use with hardware kit 820400-901 Phase: GF-250 Molecular Weight Lower Limit: 4000 Da Molecular Weight Upper Limit: 400000 Da Hardware: SS Inner Diameter (ID): 21.2 mm Length: 250 mm Particle Size: 6 µm See all product information Est. ship date 4 July
884973-701	ZORBAX GF-250, 4.6 x 250 mm, 4 µm	SS	4.6 mm	250 mm	4 µm	GF-250	1 Each	Request Quote	Quantity
ZORBAX GF-250, 4.6 x 250 mm, 4 µm Phase: GF-250 Molecular Weight Lower Limit: 4000 Da Molecular Weight Upper Limit: 400000 Da Hardware: SS Inner Diameter (ID): 4.6 mm Length: 250 mm Particle Size: 4 µm See all product information Est. ship date 4 July
884973-761	ZORBAX GF-250, 4.6 x 250 mm, 6 µm	SS	4.6 mm	250 mm	6 µm	GF-250	1 Each	Request Quote	Quantity
ZORBAX GF-250, 4.6 x 250 mm, 6 µm Phase: GF-250 Molecular Weight Lower Limit: 4000 Da Molecular Weight Upper Limit: 400000 Da Hardware: SS Inner Diameter (ID): 4.6 mm Length: 250 mm Particle Size: 6 µm See all product information Est. ship date 4 July
884973-901	ZORBAX GF-250, 9.4 x 250 mm, 4 µm	SS	9.4 mm	250 mm	4 µm	GF-250	1 Each	Request Quote	Quantity
ZORBAX GF-250, 9.4 x 250 mm, 4 µm Phase: GF-250 Molecular Weight Lower Limit: 4000 Da Molecular Weight Upper Limit: 400000 Da Hardware: SS Inner Diameter (ID): 9.4 mm Length: 250 mm Particle Size: 4 µm See all product information
820212-911	ZORBAX GF-450, 15.8 x 10 mm, guard cartridge (PrepHT), 5 µm, 2/pk	SS	17 mm	8 mm	5 µm	GF-450	2 Pack	Request Quote	Quantity
ZORBAX GF-450, 15.8 x 10 mm, guard cartridge (PrepHT), 5 µm, 2/pk. Use with end-fitting 820444-901, or standalone hardware kit 420420-901. Phase: GF-450 Guard Column: Yes Molecular Weight Lower Limit: 10000 Da Molecular Weight Upper Limit: 900000 Da Hardware: SS Inner Diameter (ID): 17 mm Length: 8 mm Particle Size: 5 µm See all product information Est. ship date 4 July

1 - 12 of 14 results

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Product Details

Features

High efficiency and reproducibility with short analysis time
Semi-prep and prep column dimensions
Compatible with organic modifiers and denaturants
Wide usable pH range (3-8)

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Filter by

Yes

Kit

4.6 mm

12.5 mm

21.2 mm

Yes

9.4 mm

15 mm

Yes

4.6 mm

13 mm

6 µm

GF-250 Diol

Yes

0 Da

3000 Da

Cartridge

9.4 mm

15 mm

6 µm

GF-250

4000 Da

400000 Da

SS

21.2 mm

250 mm

6 µm

GF-250

4000 Da

400000 Da

SS

4.6 mm

250 mm

4 µm

GF-250

4000 Da

400000 Da

SS

4.6 mm

250 mm

6 µm

GF-250

4000 Da

400000 Da

SS

9.4 mm

250 mm

4 µm

GF-450

Yes

10000 Da

900000 Da

SS

17 mm

8 mm

5 µm

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